ROS Detection Cell-Based Assay Kit (DHE) | Cayman Chemical
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ROS Detection Cell-Based Assay Kit (DHE)

Item № 601290
product image
     96 wells $165.00 0.00

Pricing updated 2018-12-19. Prices are subject to change without notice.

  • Measures ROS directly in live cells
  • Specific for superoxide and hydrogen peroxide
  • Includes positive control for ROS generation and negative control for ROS scavenging
  • Flow cytometry and plate-based methods
  • Reactive Oxidation Species CBA Kit (dihydroethidium)

Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to form ethidium (E+) (ex 480 nm/em 576 nm).1,2 Given their narrow spectral range, distinguishing between the two species using filter-based optical systems is often difficult and can lead to misreporting of the species of ROS being generated. This assay kit uses DHE as a fluorescent probe for the detection of ROS generation. Antimycin A, an inhibitor of complex III of the mitochondrial electron transport chain, is included as a positive control for ROS generation. N-acetyl Cysteine is included as an antioxidant control.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Reactive Oxidation Species CBA Kit (dihydroethidium)

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Michalski, R., Michalowski, B., Sikora, A., et al. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: A reassessment Free Redic. Biol. Med. 67, 278-284 (2014).

2. Zhao, H., Kalivendi, S., Zhang, H., et al. Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: Potential implications in intracellular fluorescence detection of superoxide Free Radic. Biol. Med. 34(11), 1359-1368 (2003).

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You may be eligible to receive a free sample of ROS Detection Cell-Based Assay Kit (DHE) under the Cayman Challenge program.

Cayman Chemical

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Ann Arbor, Michigan 48108 USA

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