Myeloperoxidase Peroxidation Fluorometric Assay Kit

Item № 700160
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
2 x 96 wells $219.00 0.00

Pricing updated 2018-02-22. Prices are subject to change without notice.

Description
Features
  • Measure MPO peroxidase activity in cell lysates and purified preparations
  • Assay 38 samples in duplicate
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm)
Synonyms
  • MPO Peroxidation Assay Kit

Myeloperoxidase (MPO) is a member of the heme peroxidase superfamily and is stored within the azurophilic granules of leukocytes.1 MPO is found within circulating neutrophils, monocytes, and some tissue macrophages.2 A unique activity of MPO is its ability to use chloride as a cosubstrate with hydrogen peroxide to generate chlorinating oxidants such as hypochlorous acid, a potent antimicrobial agent.3 Recently, evidence has emerged that MPO-derived oxidants contribute to tissue damage and the initiation and propagation of acute and chronic vascular inflammatory diseases.4,5 The fact that circulating levels of MPO have been shown to predict risks for major adverse cardiac events and that levels of MPO-derived chlorinated compounds are specific biomarkers for disease progression, has attracted considerable interest in the development of therapeutically useful MPO inhibitors.6 MPO also oxidizes a variety of substrates, including phenols and anilines, via the classic peroxidation cycle. The relative concentrations of chloride and the reducing substrate determine whether MPO uses hydrogen peroxide for chlorination or peroxidation. Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530-540 nm and emission wavelength of 585-595 nm. The kit includes a MPO-specific inhibitor for distinguishing between MPO activity from MPO-independent fluorescence.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • MPO Peroxidation Assay Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Yamada, M., and Kurahashi, K. Regulation of myeloperoxidase gene expression during differentiation of human myeloid leukemia HL-60 cells. The Journal of Biological Chemisty 259(5), 3021-3025 (1984).

2. Schultz, J., and Kaminker, K. Myeloperoxidase of the leucocyte of normal human blood.1 I. Content and localization. Archives of Biochemistry and Biophysics 96, 465-467 (1962).

3. Harrison, J.E., and Schultz, J. Studies on the chlorinating activity of myeloperoxidase. The Journal of Biological Chemisty 251(5), 1371-1374 (1976).

4. Podrez, E.A.,Abu-Soud, H.M., and Hazen, S.L. Myeloperoxidase-generated oxidants and atherosclerosis. Free Radical Biology & Medicine 28(12), 1717-1725 (2000).

5. Zhang, R.,Brennan, M.L.,Fu, X., et al. Association between myeloperoxidase levels and risk of coronary artery disease. Journal of the American Medical Association 286(17), 2136-2142 (2001).

6. Malle, E.,Furtmüller, P.G.,Sattler, W., et al. Myeloperoxidase: A target for new drug development? British Journal of Pharmacology 152, 838-854 (2007).

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You may be eligible to receive a free sample of Myeloperoxidase Peroxidation Fluorometric Assay Kit under the Cayman Challenge program.

Cayman Chemical

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Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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