β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit | Cayman Chemical
We collect cookies for vital website function and to better serve our customers. By continuing to browse you agree to the storing of cookies on your device. See our privacy policy for details.

β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

Item № 700190
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $225.00 0.00

Pricing updated 2018-09-23. Prices are subject to change without notice.

Description
Features
  • Measure β-HB from plasma, serum, urine, cell lysates, and tissue homogenates
  • Assay 40 samples in duplicate
  • Assay Range: 25-500 μM
  • Plate-based colorimetric measurement (445-455 nm)
Synonyms
  • β-HB
  • 3-Hydroxybutyric Acid

β-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is a “ketone body” which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. The term ‘ketone body’ refers to three molecules, acetoacetate, β-HB, and acetone. β-HB and acetoacetate transport energy from the liver to the other tissues and acetone is generated by spontaneous decarboxylation of acetoacetate.1 The presence of ketosis may be normal or pathologic. Normally ketosis can indicate that lipid metabolism has been activated and the pathway of lipid degradation is intact. Normal ketosis is prevalent in many circumstances such as during fasting, after prolonged exercise or after a high fat diet. Pathological causes of ketosis include multiple organ failure, diabetes, childhood hypoglycemia, corticosteroid or growth hormone deficiency, intoxication with alcohol or salicylates and several inborn errors of metabolism.2 In acutely ill patients, these ketone bodies can accumulate in the body to cause ketoacidosis, which leads to the potentially life threatening condition known as metabolic acidosis.3 The presence and degree of ketosis can be determined by measuring blood levels of β-HB. Ordinarily, β-HB accounts for approximately 75% of the ketone bodies in serum.4,5,6 Measurement of β-HB provides a reliable index of the level of ketoacidosis, including the detection of subclinical ketosis.7,8,9 In diabetics, β-HB measurements (and blood glucose) can be used for the assessment of the severity of diabetic coma and is essential for the exclusion of hyperosmolar non-ketotic diabetic coma. The measurement of β-HB is also used to monitor insulin requirements, based on existing hyperketonemia.10 β-HB has more recently been evaluated for use in neurodegenerative diseases and inhibition of adipocyte lipolysis.11,12,13,14,15 Cayman’s β-HB (Ketone Body) Assay Kit provides a simple, reproducible, and sensitive tool for measuring β-HB levels in plasma, serum, urine, cell lysates, or tissue homogenates. The method for β-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. In the presence of diaphorase, NADH reacts with the colorimetric detector WST-1 to produce a formazan dye with an absorbance maximum at 445-455 nm. The absorbance of the dye is directly proportional to the β-HB concentration.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Recommended Products

Related Products
View Related Product Categories
Technical Information
Synonyms
  • β-HB
  • 3-Hydroxybutyric Acid

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. . . .

2. . . .

3. . . .

4. . . .

5. . . .

6. . . .

7. . . .

8. . . .

9. . . .

10. . . .

11. . . .

12. . . .

13. . . .

14. . . .

15. . . .

16. . . .

Technical Support
Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We work with scientists to support their research, drug discovery, and drug development needs through our expertise in the following research services:

Promotions

View all of our current Promotions

You may be eligible to receive a free sample of β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640