β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit | Cayman Chemical
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β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

Item № 700190
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $225.00 0.00

Pricing updated 2018-12-19. Prices are subject to change without notice.

Description
Features
  • Measure β-HB from plasma, serum, urine, cell lysates, and tissue homogenates
  • Assay 40 samples in duplicate
  • Assay Range: 25-500 μM
  • Plate-based colorimetric measurement (445-455 nm)
Synonyms
  • β-HB
  • 3-Hydroxybutyric Acid

β-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is a “ketone body” which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. The term ‘ketone body’ refers to three molecules, acetoacetate, β-HB, and acetone. β-HB and acetoacetate transport energy from the liver to the other tissues and acetone is generated by spontaneous decarboxylation of acetoacetate.1 The presence of ketosis may be normal or pathologic. Normally ketosis can indicate that lipid metabolism has been activated and the pathway of lipid degradation is intact. Normal ketosis is prevalent in many circumstances such as during fasting, after prolonged exercise or after a high fat diet. Pathological causes of ketosis include multiple organ failure, diabetes, childhood hypoglycemia, corticosteroid or growth hormone deficiency, intoxication with alcohol or salicylates and several inborn errors of metabolism.2 In acutely ill patients, these ketone bodies can accumulate in the body to cause ketoacidosis, which leads to the potentially life threatening condition known as metabolic acidosis.3 The presence and degree of ketosis can be determined by measuring blood levels of β-HB. Ordinarily, β-HB accounts for approximately 75% of the ketone bodies in serum.4,5,6 Measurement of β-HB provides a reliable index of the level of ketoacidosis, including the detection of subclinical ketosis.7,8,9 In diabetics, β-HB measurements (and blood glucose) can be used for the assessment of the severity of diabetic coma and is essential for the exclusion of hyperosmolar non-ketotic diabetic coma. The measurement of β-HB is also used to monitor insulin requirements, based on existing hyperketonemia.10 β-HB has more recently been evaluated for use in neurodegenerative diseases and inhibition of adipocyte lipolysis.11,12,13,14,15 Cayman’s β-HB (Ketone Body) Assay Kit provides a simple, reproducible, and sensitive tool for measuring β-HB levels in plasma, serum, urine, cell lysates, or tissue homogenates. The method for β-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. In the presence of diaphorase, NADH reacts with the colorimetric detector WST-1 to produce a formazan dye with an absorbance maximum at 445-455 nm. The absorbance of the dye is directly proportional to the β-HB concentration.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • β-HB
  • 3-Hydroxybutyric Acid

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Guthrie, J.P., and Jordan, F. Amine-catalyzed decarboxylation of acetoacetic acid. The rate constant for decarboxylation of a β-imino acid Journal of the American Chemical Society 94(26), 9136-9141 (1972).

2. Galán, A., Hernández, J.M., and Jimenez, O. Measurement of blood acetoacetate and β-hydroxybutyrate in an automatic analyser Journal of Automated Methods & Management in Chemistry 23(3), 69-76 (2001).

3. Foster, D.W., and McGarry, J.D. The metabolic derangements and treatment of diabetic ketoacidosis New England Journal of Medicine 309(3), 159-169 (1983).

4. Persson, B. Determination of plasma acetoacetate and D-ß-hydroxy-butyrate in new-born infants by an enzymatic fluorometric micro-method Scandinavian Journal of Clinical and Laboratory Investigation 25(1), 9-18 (1970).

5. Wildenhoff, K.E. A micro-method for the enzymatic determination of acetoacetate and 3-hydroxybutyrate in blood and urine Scandinavian Journal of Clinical and Laboratory Investigation 25(2), 171-179 (1970).

6. Koch, D.D., and Feidbruegge, H. Optimized kinetic method for automated determination of β-hydroxybutyrate Clinical Chemistry 33(10), 1761-1766 (1987).

7. Li, P.K., Lee, J.T., MacGillivray, M.H., et al. Direct, fixed-time kinetic assays for β-hydroxybutyrate and acetoacetate with a centrifugal analyzer or a computer-backed spectrophotometer Clinical Chemistry 26(12), 1713-1717 (1980).

8. Harano, Y., Kosugi, K., Hyosu, T., et al. Sensitive and simplified method for the differential determination of serum levels of ketone bodies Clinica Chimica Acta 134, 327-336 (1983).

9. MacGillivray, M.H., Li, P.K., Lee, J.T., et al. Elevated plasma ß-hydroxybutyrate concentrations without ketonuria in healthy insulin-dependent diabetic patients Journal of Clinical Endocrinology and Metabolism 54(3), 665-668 (1982).

10. Alberti, K.G.M.M., and Hockaday, T.D.R. Rapid blood ketone body estimation in the diagnosis of diabetic ketoacidosis British Medical Journal 2, 565-568 (1972).

11. Kashiwaya, Y., Takeshima, T., Mori, N., et al. D-β-hydroxybutyrate protects neurons in models of Alzheimer’s and Parkinson’s disease Proceedings of the National Academy of Sciences of the United States of America 97(10), 5440-5444 (2000).

12. Tieu, K., Perier, C., Caspersen, C., et al. D-β-hydroxybutyrate rescues mitochondrial respiration and mitigates features of Parkinson disease Journal of Clinical Investigation 112(6), 892-901 (2003).

13. Reger, M.A., Henderson, S.T., Hale, C., et al. Effects of β-hydroxybutyrate on cognition in memory-impaired adults Neurobiology of Aging 25, 311-314 (2004).

14. Cheng, B., Yang, X., Hou, Z., et al. D-β-hydroxybutyrate inhibits the apoptosis of PC12 cells induced by 6-OHDA in relation to up-regulating the ratio of Bcl-2/Bax mRNA Autonomic Neuroscience: Basic and Clinical 134, 38-44 (2007).

15. Taggart, A.K.P., Kero, J., Gan, X., et al. (D)-β-hydroxybutyrate inhibits adipocyte lipolysis via the nicotinic acid receptor PUMA-G The Journal of Biological Chemisty 280(29), 26649-26652 (2005).

16. McMurray, C.H., Blanchflower, W.J., and Rice, D.A. Automated kinetic method for D-3-hydroxybutyrate in plasma or serum Clinical Chemistry 30(3), 421-425 (1984).

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