COX Fluorescent Activity Assay Kit | Cayman Chemical
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COX Fluorescent Activity Assay Kit

Item № 700200
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     2 x 96 wells $375.00 0.00

Pricing updated 2018-11-19. Prices are subject to change without notice.

Description
Features
  • Measure COX activity in cell lysates, tissue homogenates, and purified enzyme preparations
  • Measure the peroxidase component of the COX
  • Assay 70 samples in duplicate
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm)
  • Includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity
Synonyms
  • Cyclooxygenase Fluorescent Activity Assay Kit

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both COX and peroxidase activities. The COX component converts arachidonic acid to a hydroperoxy endoperoxide (PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (PGH2), the precursor of PGs, thromboxanes, and prostacyclins.1,2 It is now well established that there are two distinct isoforms of COX, namely COX-1 and COX-2. Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin that can be analyzed using an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Cyclooxygenase Fluorescent Activity Assay Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Nugteren, D.H., and Hazelhof, E. Isolation and properties of intermediates in prostaglandin biosynthesis Biochimica et Biophysica Acta 326, 448-461 (1973).

2. Hamberg, M., and Samuelsson, B. Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis Proceedings of the National Academy of Sciences of the United States of America 70, 899-903 (1973).

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You may be eligible to receive a free sample of COX Fluorescent Activity Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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Fax: (734) 971-3640