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Glutathione Assay Kit

Item № 703002
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     96 wells $230.00 0.00
     480 wells $430.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure total, oxidized (GSSG), and/or reduced (GSH) glutathione in cell lysates, tissue homogenates, plasma and erythrocyte lysates, and serum
  • Assay 40 samples in duplicate
  • Assay Range: 0.25-8 μM (GSSG) or 0.5-16 μM (GSH)
  • Plate-based colorimetric measurement (405-414 nm)
  • GSH Assay Kit

Cayman’s GSH assay kit utilizes a carefully optimized enzymatic recycling method, using glutathione reductase for the quantification of GSH.1,2,3 The sulfhydryl group of GSH reacts with DTNB (5,5'-dithio-bis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by glutathione reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to this recycling reaction which in turn is directly proportional to the concentration of GSH in the sample. Measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of GSH in the sample. GSH is easily oxidized to the disulfide dimer GSSG. Because of the use of glutathione reductase in the Cayman GSH assay kit, both GSH and GSSG are measured and the assay reflects total glutathione. The kit can also be used to measure only GSSG by following an alternative protocol. GSH measurement can be done in plasma, tissue samples, and cultured cells using this kit. Nearly all samples require deproteination before assay.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • GSH Assay Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Baker, M.A., Cerniglia, G.J., and Zaman, A. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples Analytical Biochemistry 190, 360-365 (1990).

2. Eyer, P., and Podhradsky, D. Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman’s reagent Analytical Biochemistry 153, 57-66 (1986).

3. Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues Analytical Biochemistry 27, 502-522 (1969).

Product Citations

Cao, J.Y., Poddar, A., Magtanong, L., et al. A genome-wide haploid genetic screen identifies regulators of glutathione abundance and ferroptosis sensitivity Cell Rep. 26(6), 1544-1556 (2019).

Magtanong, L., Ko, P.-J., To, M., et al. Exogenous monounsaturated fatty acids promote a ferroptosis-resistant cell state Cell Chem. Biol. 26(3), 420-432 (2019).

Wu, M., Yang, Y., Wang, M.Z., F., et al. Exogenous pancreatic kallikrein improves diabetic cardiomyopathy in streptozotocin-induced diabetes Front. Pharmcacol. 9:855, (2018).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Glutathione Assay Kit

View all analysis workbooks

Contact Us
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  • Direct Phone: (734) 975-3888
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