We collect cookies for vital website function and to better serve our customers. By continuing to browse you agree to the storing of cookies on your device. See our privacy policy for details.

Glutathione Peroxidase Assay Kit

Item № 703102
product image
     96 wells $285.00 0.00
     480 wells $490.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Measure GPX in plasma, erythrocyte lysate, tissue homogenates, and cell lysates
  • Assay 45 samples in duplicate
  • Measure GPX activity down to 50 U/ml
  • Plate-based colorimetric measurement (340 nm)
  • GPX Assay Kit

Glutathione peroxidase (GPX) catalyzes the reduction of hydroperoxides, including hydrogen peroxides, by reduced glutathione and functions to protect the cell from oxidative damage. With the exception of phospholipid-hydroperoxide GPX, a monomer, all of the GPX enzymes are tetramers of four identical subunits.1,2 Each subunit contains a selenocysteine in the active site which participates directly in the two-electron reduction of the peroxide substrate.1,2 The enzyme uses glutathione as the ultimate electron donor to regenerate the reduced form of the selenocysteine.1,2 The Cayman Chemical Glutathione Peroxidase Assay Kit measures GPX activity indirectly by a coupled reaction with glutathione reductase (GR). Oxidized glutathione (GSSG), produced upon reduction of an organic hydroperoxide by GPX, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the A340 is directly proportional to the GPX activity in the sample.3 The Cayman GPX Assay Kit can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Recommended Products

Related Products
View Related Product Categories
Technical Information
  • GPX Assay Kit
  • Animal/Bovine

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Ceballos-Picot, I., Trivier, J.M., Nicole, A., et al. Age-correlated modifications of copper-zinc superoxide dismutase and glutathione-related enzyme activities in human erythrocytes Clinical Chemistry 38, 66-70 (1992).

2. Forstrom, J.W., Zakowski, J.J., and Tappel, A.L. Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine Biochemistry 17, 2639-2644 (1978).

3. Paglia, D.E., and Valentine, W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase Journal of Laboratory and Clinical Medicine 70, 158-169 (1967).

Product Citations

Arazi, H., Mohammadjafari, H., and Asadi, A. Use of anabolic androgenic steroids produces greater oxidative stress responses to resistance exercise in strength-trained men Toxicol. Rep. 4, 282-286 (2017).

Zadeh-Ardabili, P.M., Rad, S.K., Rad, S.K., et al. Palm vitamin E reduces locomotor dysfunction and morphological changes induced by spinal cord injury and protects against oxidative damage Sci. Rep. 7(14365), (2017).

Liu, X., Zhang, S., Whitowrth, R.J., et al. Unbalanced activation of glutathione metabolic pathways suggests potential involvement in plant defense against the gall midge Mayetiola destructor in wheat Sci. Rep. 5:8092, (2015).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Glutathione Peroxidase Assay Kit

View all analysis workbooks

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

We work with scientists to accelerate their research, drug discovery, and drug development needs through our expertise in the following areas:

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640