Glutathione S-Transferase Assay Kit

Item № 703302
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 wells $225.00 0.00

Pricing updated 2018-02-18. Prices are subject to change without notice.

Description
Features
  • Measure GST activity in plasma, tissue homogenates, and cell lysates
  • Assay 22 samples in duplicate
  • Measure GST activity down to 24 U/ml
  • Plate-based colorimetric measurement (340 nm)
Synonyms
  • GST Assay Kit

Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble.1 The glutathione conjugates are metabolized further to mercapturic acid and then excreted.1 Based on their sequence homology, substrate specificity, and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes.2,3,4,5 These classes are comprised of cytosolic enzymes and the fifth form is microsomal.2,3,4,5 The Cayman Chemical Glutathione S-Transferase Assay Kit measures total GST activity (cytosolic and microsomal) by measuring the conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione.6 The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample. The Cayman GST Assay Kit can be used to measure GST activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Cytosolic and microsomal GST activity can also be assayed separately.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • GST Assay Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Boyland, E., and Chasseaud, L.F. The role of glutathione and glutathione S-transferases in mercapturic acid biosynthesis. Advances in Enzymology 32, 173-219 (1969).

2. Mannervik, B. The isoenzymes of glutathione transferase. Advances in Enzymology and Related Areas of Molecular Biology 57, 357-417 (1985).

3. Mannervik, B.,Ålin, P.,Guthenberg, C., et al. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: Correlation between structural data and enzymatic properties. Proceedings of the National Academy of Sciences of the United States of America 82, 7202-7206 (1985).

4. Morgenstern, R.,DePierre, J.W., and Ernster, L. Activation of microsomal glutathione S-transferase activity by sulfhydryl reagents. Biochemical and Biophysical Research Communications 87, 657-663 (1979).

5. Jakoby, W.B. The glutathione S-transferases: A group of multifunctional detoxification proteins. Advances in Enzymology and Related Areas of Molecular Biology 46, 383-414 (1978).

6. Habig, W.H.,Pabst, M.J., and Jakoby, W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemisty 249, 7130-7139 (1974).

Technical Support
Analysis Tools

View our analysis workbook for Glutathione S-Transferase Assay Kit

View all analysis workbooks

FAQ

Can the Glutathione S-Transferase Assay Kit kit be used for porcine samples?

This kit has not been tested specifically with porcine samples, but the assay should work with all GSTs. Since GSTs are highly conserved enzymes, this kit should work well for samples from any species.

What type of plasma can be used in the Glutathione S-Transferase Assay Kit?

Any of the anticoagulants used in plasma such as heparin, citrate, or EDTA are suitable for the Glutathione S-Transferase Assay Kit.

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  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Glutathione S-Transferase Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640