Pricing updated 2018-02-20. Prices are subject to change without notice.
Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.1,2,3 Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.4 An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is designed for use with either a single-
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.
1. Cross, C.E.,Halliwell, B.,Borish, E.T., et al. Oxygen radicals and human disease. Annals of Internal Medicine 107, 526-545 (1987).
2. Halliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radical Research 25, 57-74 (1996).
3. Porter, N.A.,Mills, K.A. and Caldwell, S.E. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30, 277-290 (1995).
4. Roomi, M.W. and Hopkins, C.Y. Some reactions of sterculic and malvalic acids. A new source of malvalic acid. Canadian Journal of Biochemistry 48, 759-762 (1970).
Are serum samples suitable for the Lipid Hydroperoxide (LPO) Assay Kit ?
Yes, Serum samples are fine for the LPO Assay Kit. In the kit booklet there is a procedure in the Sample Preparation for plasma which is suitable for serum also.
Is there an optimized sample concentration or tissue weight for the Lipid Hydroperoxide (LPO) Assay Kit ?
In the Lipid Hydroperoxide (LPO) Assay Kit booklet you are directed to use a known amount of sample and an equal volume of Extract R saturated methanol. Unfortunately we do not have values for quantities of tissue samples to suggest. There are so many tissue types that it would be difficult to suggest quantities. We suggest that in the course of your experiment you try a few different quantities and dilutions to optimize the concentration.
If the level of LPO in the samples is low how is this handeled with Lipid Hydroperoxide (LPO) Assay Kit?
With the Lipid Hydroperoxide (LPO) Assay Kit there is no procedure for concentrating the sample. When the level of lipid peroxides in a sample is below the detection limit of the LPO Assay, and a more sensitive assay for lipid oxidation is required. In these cases we suggest using the 8-Isoprostane EIA Kit, item # 516351, which assess the levels of a stable end product of lipid oxidation.