Lipid Hydroperoxide (LPO) Assay Kit (96 well) | Cayman Chemical

Lipid Hydroperoxide (LPO) Assay Kit (96 well)

Item № 705003
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $385.00 0.00

Pricing updated 2018-05-24. Prices are subject to change without notice.

Description
Features
  • Measure LPOs in tissues, cultured cells, plant materials, foods, and biological fluids
  • Assay 40 samples in duplicate
  • Assay Range: 0.25-5 nmol
  • Plate-based colorimetric measurement (500 nm)
  • Includes reusable glass plate

Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.1,2,3 Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.4 An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is available in two formats: 100 dtn, which is designed to be read using a single-tube spectrophotometer, and 96 wells, which is designed to be read using a 96-well microplate reader. The 96 well kit contains a special glass plate which is resistant to organic solvents used in the assay; this is the only difference between the two kit types.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Cross, C.E., Halliwell, B., Borish, E.T., et al. Oxygen radicals and human disease. Annals of Internal Medicine 107, 526-545 (1987).

2. Halliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radical Research 25, 57-74 (1996).

3. Porter, N.A., Mills, K.A., and Caldwell, S.E. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30, 277-290 (1995).

4. Roomi, M.W., and Hopkins, C.Y. Some reactions of sterculic and malvalic acids. A new source of malvalic acid. Canadian Journal of Biochemistry 48, 759-762 (1970).

Technical Support
FAQ

Are serum samples suitable for Lipid Hydroperoxide (LPO) Assay Kit?

Yes, serum samples may be suitable for the LPO Assay Kit. In the kit booklet there is a procedure in the Sample Preparation for plasma which is suitable for serum also.

Is there a protocol for serum sample preparation for the Lipid Hydroperoxide (LPO) Assay Kit?

In the Lipid Hydroperoxide (LPO) Assay Kit booklet there is a procedure for sample preparation for plasma which is suitable for serum also.

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You may be eligible to receive a free sample of Lipid Hydroperoxide (LPO) Assay Kit (96 well) under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640