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Catalase Assay Kit

Item № 707002
product image
     96 wells $270.00 0.00
     480 wells $490.00 0.00

Pricing updated 2019-06-15. Prices are subject to change without notice.

  • Measure catalase activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates
  • Assay 40 samples in duplicate
  • Measure catalase activity down to 2 U/ml
  • Plate-based colorimetric measurement (540 nm)
  • CAT

Catalase (EC; 2H2O2 oxidoreductase) is an ubiquitous antioxidant enzyme that is present in most aerobic cells. Catalase (CAT) is involved in the detoxification of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), which is a toxic product of both normal aerobic metabolism and pathogenic ROS production. This enzyme catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While the aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. In humans, the highest levels of catalase are found in liver, kidney, and erythrocytes, where it is believed to account for the majority of hydrogen peroxide decomposition. The Cayman Chemical Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured spectrophotometrically with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald) as the chromogen.1,2 Purpald specifically forms a bicyclic heterocycle with aldehydes, which upon oxidation changes from colorless to a purple color.1,2 The assay can be used to measure CAT activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • CAT
  • Animal/Bovine

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Johansson, L.H., and Borg, L.A.H. A spectrophotometric method for determination of catalase activity in small tissue samples Analytical Biochemistry 174, 331-336 (1988).

2. Wheeler, C.R., Salzman, J.A., Elsayed, N.M., et al. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity Analytical Biochemistry 184, 193-199 (1990).

Product Citations

Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of Gluteus Medius muscle in goats PLOS One 11(5), e0154603 (2016).

Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats J. Anim. Sci. Technol. 58(23), (2016).

Vitti, A., Pellegrini, E., Nali, C., et al. Trichoderma harzianum T-22 induces systemic resistance in tomato infected by Cucumber mosaic virus Front. Plant Sci. 7(1520), (2016).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Catalase Assay Kit

View all analysis workbooks

View the pre-configured analysis tool for Catalase Assay Kit on MyAssays.com

Contact Us
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  • Direct Phone: (734) 975-3888
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Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640