Antioxidant Assay Kit | Cayman Chemical
We collect cookies for vital website function and to better serve our customers. By continuing to browse you agree to the storing of cookies on your device. See our privacy policy for details.

Antioxidant Assay Kit

Item № 709001
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $230.00 0.00

Pricing updated 2018-11-15. Prices are subject to change without notice.

Description
Features
  • Measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates
  • Assay 41 samples in duplicate
  • Measure antioxidant capacity in Trolox equivalents down to 44 μM
  • Plate-based colorimetric measurement (750 or 405 nm)

The antioxidant system of living organisms includes enzymes such as superoxide dismutase, catalase, and glutathione peroxidase; macromolecules such as albumin, ceruloplasmin, and ferritin; and an array of small molecules, including ascorbic acid, α-tocopherol, β-carotene, reduced glutathione, uric acid, and bilirubin. The sum of endogenous and food-derived antioxidants represents the total antioxidant activity of the extracellular fluid. Cooperation of all the different antioxidants provides greater protection against attack by reactive oxygen or nitrogen radicals, than any single compound alone. Thus, the overall antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present in plasma and body fluids. The Cayman Chemical Antioxidant Assay Kit measures the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2'-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS® · + by metmyoglobin.1,2,3,4 The capacity of the antioxidants in the sample to prevent ABTS oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as molar Trolox equivalents. Each kit contains assay buffer (10X), chromogen, metmyoglobin, trolox, hydrogen peroxide, a 96 well plate, plate cover, and complete instructions.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Recommended Products

Related Products
View Related Product Categories
Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Miller, N.J., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates Clinical Science 84, 407-412 (1993).

2. Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS + radical cation assay Free Radical Research 26, 195-199 (1997).

3. Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity Biochemical Society Transactions 21, 95S-95S (1993).

4. Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids Methods in Enzymology 234(24), 279-293 (1994).

Product Citations

Peng, K.-T., Tsai, M.-H., Lee, C.-W., et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis PLOS One 13(8), e0202501 (2018).

Tsamesidis, I., Fozza, C., Vagdatli, E., et al. Total antioxidant capacity in Mediterranean β-thalassemic patients Adv. Clin. Exp. Med. 26(5), 789-793 (2017).

Technical Support
Analysis Tools

View our analysis workbook for Antioxidant Assay Kit

View all analysis workbooks

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

We work with scientists to accelerate their research, drug discovery, and drug development needs through our expertise in the following areas:

Promotions

View all of our current Promotions

You may be eligible to receive a free sample of Antioxidant Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640