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Antioxidant Assay Kit

Item № 709001
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     96 wells $230.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates
  • Assay 41 samples in duplicate
  • Measure antioxidant capacity in Trolox equivalents down to 44 μM
  • Plate-based colorimetric measurement (750 or 405 nm)

The antioxidant system of living organisms includes enzymes such as superoxide dismutase, catalase, and glutathione peroxidase; macromolecules such as albumin, ceruloplasmin, and ferritin; and an array of small molecules, including ascorbic acid, α-tocopherol, β-carotene, reduced glutathione, uric acid, and bilirubin. The sum of endogenous and food-derived antioxidants represents the total antioxidant activity of the extracellular fluid. Cooperation of all the different antioxidants provides greater protection against attack by reactive oxygen or nitrogen radicals, than any single compound alone. Thus, the overall antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present in plasma and body fluids. The Cayman Chemical Antioxidant Assay Kit measures the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2'-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS® · + by metmyoglobin.1,2,3,4 The capacity of the antioxidants in the sample to prevent ABTS oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as molar Trolox equivalents. Each kit contains assay buffer (10X), chromogen, metmyoglobin, trolox, hydrogen peroxide, a 96 well plate, plate cover, and complete instructions.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Animal/Horse

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Miller, N.J., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates Clinical Science 84, 407-412 (1993).

2. Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS + radical cation assay Free Radical Research 26, 195-199 (1997).

3. Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity Biochemical Society Transactions 21, 95S-95S (1993).

4. Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids Methods in Enzymology 234(24), 279-293 (1994).

Product Citations

Wen, J.J., Yin, Y.W., and Garg, N.J. PARP1 depletion improves mitochondrial and heart function in Chagas disease: Effects on POLG dependent mtDNA maintenance PLoS Pathog. 14(5), e1007065 (2018).

Peng, K.-T., Tsai, M.-H., Lee, C.-W., et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis PLOS One 13(8), e0202501 (2018).

Vieira, G.L.T., Lossie, A.C., Lay, D.C., Jr., et al. Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania PLoS One 12(4), e0175222 (2017).

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Technical Support
Analysis Tools

View our analysis workbook for Antioxidant Assay Kit

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Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

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Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640