COX Activity Assay Kit | Cayman Chemical

COX Activity Assay Kit

Item № 760151
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $375.00 0.00

Pricing updated 2018-05-20. Prices are subject to change without notice.

Description
Features
  • Measure COX activity in cell lysates, tissue homogenates, and purified enzyme preparations
  • Measure the peroxidase component of the COX
  • Assay 36 samples in duplicate
  • Plate-based colorimetric measurement (590 nm)
  • Includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity

The COX Activity Assay utilizes the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 The assay can be used to measure COX activity (COX-1 and COX-2) in cell lysates, tissue homogenates, and purified enzyme preparations. The assay includes COX-1 and COX-2 specific inhibitors in order to distinguish between the two isozymes.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25, 531-540 (1983).

Technical Support
FAQ

In the COX Activity Assay Kit, what is the principle of the reaction of TMPD by peroxidase?

In the Background section of the COX Activity Assay Kit booklet, it states that the COX component converts arachidonic acid to Prostaglandin G2 (PGG2) and the peroxidase component reduces the PGG2 to the corresponding alcohol, PGH2. In this kit the TMPD serves as a reducing agent, TMPD gets oxidized, so electrons flow from the TMPD to PGG2 and the appearance of the oxidized. TMPD is monitored at 590 nm.

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You may be eligible to receive a free sample of COX Activity Assay Kit under the Cayman Challenge program.

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