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Nitrate/Nitrite Colorimetric Assay Kit

Item № 780001
product image
     2 x 96 wells $185.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure nitrate, nitrite, and/or total nitrate/nitrites in plasma/serum, urine, tissue culture media, and tissue homogenates
  • Assay 40 samples in duplicate/96-well plate
  • Assay Range: 5-35 µM
  • Plate-based colorimetric measurement (540-550 nm)
  • Nitric Oxide Metabolite Detection Kit

The Cayman Chemical Nitrate/Nitrite Assay Kit provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step is the addition of the Griess Reagents which convert nitrite into a deep purple azo compound. Photometric measurement of the absorbance due to this azo chromophore accurately determines nitrite concentration. This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media, and tissue homogenates. However, it cannot be used to analyze nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH has been added. For these assays a second method (LDH method) is utilized and is available from Cayman Chemical in a 96 well plate format (Item No. 760871).

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Nitric Oxide Metabolite Detection Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Ferreira, M.S., Aride, P.H.R., and Val, A.L. Could resistance to lactate accumulation contribute to the better swimming performance of Brycon amazonicus when compared to Colossoma macropomum? Peer J. 6, e5719 (2018).

Wu, M., Yang, Y., Wang, M.Z., F., et al. Exogenous pancreatic kallikrein improves diabetic cardiomyopathy in streptozotocin-induced diabetes Front. Pharmcacol. 9:855, (2018).

Tarabová, B., Lukeš, P., Janda, M., et al. Specificty of detection methods of nitrites and ozone in aqueous solutions activated by air plasma Plasma Process. Polymer. 15, e1800030 (2018).

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Technical Support

What are the main differences between the Nitrate/Nitrite Colorimetric Assay Kit and Nitrate/Nitrite Colorimetric Assay Kit (LDH method)?

The Nitrate/Nitrite Colorimetric Assay , product number 780001, uses a NADPH cofactor recycling method which allows use of a NADPH concentration that is below the level of interference in the Griess reaction. The Nitrate/Nitrite Colorimetric Assay (LDH Method), product number 790871 uses LDH to eliminate all excess NADPH that is required for NOS activity assays. The LDH kit also removes excess NADPH used to catalyze conversion of Nitrate to Nitrite. Of the two nitrate/nitrite assays mentioned, we recommend the Nitrate/Nitrite Colorimetric Assay (or the corresponding Fluorometric assay if greater sensitivity is needed) for most applications since it is significantly easier to perform. The only reason to use the Nitrate/Nitrite Colorimetric Assay Kit (LDH method) is for measurement of Nitrate/nitrite from in vitro NOS activity assays.

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Ann Arbor, Michigan 48108 USA

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