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Nitrate/Nitrite Fluorometric Assay Kit

Item № 780051
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     2 x 96 wells $242.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Measure nitrate, nitrite, and/or total nitrate/nitrites in plasma, serum, tissue culture media, and tissue homogenates
  • Assay 40 samples in duplicate/96-well plate
  • Assay Range: 60 nM-3.85 μM (nitrate and/or nitrite)
  • Plate-based fluorometric measurement (ex 360-365 nm, em 430 nm)
  • Nitric Oxide Metabolite Detection Kit

Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t½ = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO interacts with the HEME prosthetic group of guanylate cyclase, activating the enzyme and leading to increased cGMP levels. Other cells (macrophages, fibroblasts, and hepatocytes) also produce NO in micromolar concentrations in response to inflammatory or mitogenic stimuli. These very high levels of NO lead to the formation of peroxynitrite, destruction of iron-sulphur clusters, thiol nitrosation, and nitration of protein tyrosine residues. Thus, the amount of NO produced in different biological systems can vary over several orders of magnitude and its subsequent chemical reactivity is diverse. The final products of NO in vivo are nitrite (NO2-) and nitrate (NO3-). Relative proportions of NO2- and NO3- within a sample are variable and cannot be predicted with certainty. Therefore, the best index of total NO production is the sum of both of these products. Cayman Chemical’s Nitrate/Nitrite Fluorometric Assay Kit provides a convenient method for the quantitation of low levels of NO2- and NO3- in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our popular colorimetric version. The minimum detectable quantity of NO2/NO3 is ~50 nM.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Nitric Oxide Metabolite Detection Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Tillmann, S., Perieira, V.S., Liebernberg, N., et al. ZL006, a small molecule inhibitor of PSD-95/nNOS interaction, does not induce antidepressant-like effects in two genetically predisposed rat models of depression and control animals PLoS One 12(8), e0182698 (2017).

Li, L., Zhu, L., Hao, B., et al. iNOS-derived nitric oxide promotes glycolysis by inducing pyruvate kinase M2 nuclear translocation in ovarian cancer Oncotarget 8(20), 33047-33063 (2017).

Jarazo Dietrich, S., Fass, M.I., Jacobo, P.V., et al. Inhibition of NOS-NO system prevents autoimmune orchitis development in rats: Relevance of NO released by testicular macrophages in germ cell apoptosis and testosterone secretion PLoS One 10(6), e0128709 (2015).

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