Nitrate/Nitrite Fluorometric Assay Kit | Cayman Chemical
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Nitrate/Nitrite Fluorometric Assay Kit

Item № 780051
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     2 x 96 wells $242.00 0.00

Pricing updated 2018-09-18. Prices are subject to change without notice.

Description
Features
  • Measure nitrate, nitrite, and/or total nitrate/nitrites in plasma, serum, tissue culture media, and tissue homogenates
  • Assay 40 samples in duplicate/96-well plate
  • Assay Range: 60 nM-3.85 μM (nitrate and/or nitrite)
  • Plate-based fluorometric measurement (ex 360-365 nm, em 430 nm)
Synonyms
  • Nitric Oxide Metabolite Detection Kit

Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t½ = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO interacts with the HEME prosthetic group of guanylate cyclase, activating the enzyme and leading to increased cGMP levels. Other cells (macrophages, fibroblasts, and hepatocytes) also produce NO in micromolar concentrations in response to inflammatory or mitogenic stimuli. These very high levels of NO lead to the formation of peroxynitrite, destruction of iron-sulphur clusters, thiol nitrosation, and nitration of protein tyrosine residues. Thus, the amount of NO produced in different biological systems can vary over several orders of magnitude and its subsequent chemical reactivity is diverse. The final products of NO in vivo are nitrite (NO2-) and nitrate (NO3-). Relative proportions of NO2- and NO3- within a sample are variable and cannot be predicted with certainty. Therefore, the best index of total NO production is the sum of both of these products. Cayman Chemical’s Nitrate/Nitrite Fluorometric Assay Kit provides a convenient method for the quantitation of low levels of NO2- and NO3- in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our popular colorimetric version. The minimum detectable quantity of NO2/NO3 is ~50 nM.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Nitric Oxide Metabolite Detection Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support
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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Nitrate/Nitrite Fluorometric Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640