We collect cookies for vital website function and to better serve our customers. By continuing to browse you agree to the storing of cookies on your device. See our privacy policy for details.

SAM-Screener Assay Technology

S-Adenosyl-L-methionine (SAM) is a co-substrate of lysine methyltransferases, utilized during the methylation of lysine residues on various proteins, including histones. SAM binds at the SET domain of methyltransferases. The SAM-Screener assays take advantage of this essential step by employing a proprietary small molecule fluorescent probe that binds to the SAM binding pocket. Binding of the probe induces a pronounced increase in fluorescence polarization. This is blocked by SAM and by the inhibitor sinefungin.

  • Directly probes the SAM binding pocket
  • Eliminates the need for difficult multi-step or radioactive enzyme assays
  • Rapidly screen large chemical libraries

Assay Set-Up. Schematic diagram of the homogenous, one-step binding assay used to probed the SAM-binding site of SET-domain methyltransferases. This assay is non-kinetic, substrate independent, and suitable for a 384- or 1536-well format.



SET7/9 SAM-Screener™ Assay Kit

Item №: 600490

G9a SAM-Screener Assay Kit

Item №: 600560 Coming soon

GLP SAM-Screener™ Assay Kit

Item №: 600570

MLL1 SAM-Screener™ Assay Kit

Item №: 600580

Discover all of our epigenetics research products.

Cayman offers contract services to screen chemical libraries, profile epigenetic enzymes, and support Hit-to-Lead discovery. Learn more about our Epigenetics Screening & Profiling Services!

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640