An inhibitor of microtubule assembly
Related Products
Isomer(s)
31419Floramultine
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Colcemid

Item No. 15364

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(7S)-6,7-dihydro-1,2,3,10-tetramethoxy-7-(methylamino)-benzo[a]heptalen-9(5H)-one
CAS Number
477-30-5
Synonyms
  • Demecolcine
  • NSC 3096
Molecular Formula
C21H25NO5
Formula Weight
371.4
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 25 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
242, 348, 352 nm
SMILES
COC1=CC=C2C([C@@H](NC)CCC3=C2C(OC)=C(OC)C(OC)=C3)=CC1=O
InChi Code
InChI=1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
InChi Key
NNJPGOLRFBJNIW-HNNXBMFYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $33

    Add

    5 mg

    $106

    Add

    10 mg

    $196

    Add

    50 mg

    $814

    Add
    Product Description

    Colcemid is a colchicine (Item No. 9000760) derivative that inhibits tubulin polymerization as potently as colchicine (IC50 = 2.1 and 2.4 μM, respectively) but is less toxic.1,2,3 At very low (nanomolar) concentrations, colcemid suppresses microtubule dynamicity and inhibits cell migration, while at micromolar levels it blocks microtubule assembly, arresting cells in metaphase.3,4,5 Mitotic block by colcemid is used to synchronize cells and for karyotyping in cytogenetic studies.4,6 Prolonged exposure to colcemid can activate p53, leading to apoptosis.7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Muzaffar, A., Brossi, A., Lin, C.M., et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J. Med. Chem. 33(2), 567-571 (1990).

    2. Rieder, C.L., and Palazzo, R.E. Colcemid and the mitotic cycle. J. Cell Sci. 102(3), 387-392 (1992).

    3. Jordan, M.A., and Wilson, L. Microtubules as a target for anticancer drugs. Nat. Rev. Cancer 4(4), 253-265 (2004).

    4. Curlej, J., Bulla, J., and Chrenek, P. Occurrence of chromosomal aneuploidy in rabbit oocytes and embryos at different developmental stages. Zygote 18(3), 203-207 (2010).

    5. Yang, H., Ganguly, A., and Cabral, F. Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs. The Journal of Biological Chemisty 285(42), 32242-32250 (2010).

    6. Bayani, J., and Squire, J.A. Preparation of cytogenetic specimens from tissue samples. Curr. Protoc. Cell Biol. 22(22.2), 1-15 (2004).

    7. Sablina, A.A., Chumakov, P.M., Levine, A.J., et al. p53 activation in response to microtubule disruption is mediated by integrin-Erk signaling. Oncogene 20(8), 899-909 (2001).