10-Acetyl-3,7-dihydroxyphenoxazine • A 6550; Amplex Red<sup>®</sup>; ADHP (CAS 119171-73-2) (Inchi key PKYCWFICOKSIHZ-UHFFFAOYSA-N) || Cayman Chemical | Supplier
10-Acetyl-3,7-dihydroxyphenoxazine
Item № 10010469
CAS № 119171-73-2
Purity ≥98%
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
1 mg $11.00 $0.00
5 mg $50.00 $0.00
10 mg $88.00 $0.00
25 mg $193.00 $0.00

Pricing updated 2015-08-28. Prices are subject to change without notice.

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Description
Synonyms
  • A 6550
  • Amplex Red®
  • ADHP

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2.1 This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.1

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Technical Information
Formal Name 1-​(3,​7-​dihydroxy-​10H-​phenoxazin-​10-​yl)-​ethanone
CAS Number 119171-73-2
Synonyms
  • A 6550
  • Amplex Red®
  • ADHP
Molecular Formula C14H11NO4
Formula Weight 257.2
Purity ≥98%
Formulation A crystalline solid
λmax 250, 285 nm
SMILES Oc1ccc2c(c1)​Oc1cc(O)​ccc1N2C(=O)​C
InChI Code ​1S/C14H11NO4/c1-8(16)​15-11-4-2-9(17)​6-13(11)​19-14-7-10(18)​3-5-12(14)​15/h2-7,17-18H,1H3
InChI Key PKYCWFICOKSIHZ-UHFFFAOYSA-N

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
Stability 2 years
Product Downloads & Resources
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Download Product Insert 105 Kb PDF

Download Safety Data Sheet (SDS) 24 Kb PDF

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Additional Information

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References & Background Reading
Product Description References

1. Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253 162-168 (1997).

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