10-Acetyl-3,7-dihydroxyphenoxazine

Cayman Chemical Item Number 10010469

A 6550; Amplex Red®; ADHP (CAS 119171-73-2)

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Description

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2.1 This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.1

1. Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253 162-168 (1997).

Synonyms
  • A 6550
  • Amplex Red®
  • ADHP
Formal Name 1-​(3,​7-​dihydroxy-​10H-​phenoxazin-​10-​yl)-​ethanone
CAS Number 119171-73-2
Molecular Formula C14H11NO4
Formula Weight 257.2
Formulation A crystalline solid
Purity ≥98%
λmax 250, 285 nm
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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Oc1ccc2c(c1)​Oc1cc(O)​ccc1N2C(=O)​C
InCHI Code
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​1S/C14H11NO4/c1-8(16)​15-11-4-2-9(17)​6-13(11)​19-14-7-10(18)​3-5-12(14)​15/h2-7,17-18H,1H3
InCHI Key
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PKYCWFICOKSIHZ-UHFFFAOYSA-N

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Size Price Quantity Subtotal
1 mg $11.00 $0.00
5 mg $50.00 $0.00
10 mg $88.00 $0.00
25 mg $193.00 $0.00
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Pricing updated 2015-03-30. Prices are subject to change without notice.

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Ann Arbor, Michigan 48108 USA

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