Estradiol EIA Kit

Cayman Chemical Item Number 582251

E2; β-Estradiol; 17β-Estradiol; 17β-Oestradiol; Estradiol ELISA Kit

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Description

Limit of detection: 80% B/B0: ~19 pg/ml · Sensitivity: 50% B/B0: ~129 pg/ml · Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.1 Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.1,5

1. Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

2. Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).

3. Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

4. Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

5. Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Synonyms
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol
  • Estradiol ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Estradiol 100%
Estradiol-3-sulfate 14.5%
Estradiol-3-glucuronide 14%
Estrone 12%
Estradiol-17-glucuronide 10%
Estriol 0.30%
5α-dihydro Testosterone 0.06%
Ethynyl Estradiol 0.05%
Aldrostenediol 0.02%
5-Androstan-17β-ol-3-one 0.02%
Aldosterone <0.01%
Cortisol <0.01%
17α-Estradiol <0.01%
Estradiol-3-benzoate <0.01%
Estradiol-17-glucoronidate <0.01%
Estradiol-17-sulfate <0.01%
Hydrocortisone <0.01%
Maturation Inducing Steroid (salmonid) <0.01%
Progesterone <0.01%
17α-hydroxy Progesterone <0.01%
Testosterone <0.01%
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Background Reading

Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).

Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

Muñoz, N.M., Kim, K.P., Han, S.K., et al. Characterization of monoclonal antibodies specific for 14-kDa human group V secretory phospholipase A2 (hVPLA2). Hybridoma 19 171-176 (2000).

Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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582251-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Estradiol AChE Tracer 100 dtn
Estradiol EIA Antiserum 100 dtn
Estradiol EIA Standard 1 ea
582251-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Estradiol AChE Tracer 100 dtn
Estradiol EIA Antiserum 100 dtn
Estradiol EIA Standard 1 ea
582251-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Estradiol AChE Tracer 500 dtn
Estradiol EIA Antiserum 500 dtn
Estradiol EIA Standard 1 ea
582251-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Estradiol AChE Tracer 500 dtn
Estradiol EIA Antiserum 500 dtn
Estradiol EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $185.00 $0.00
96 strip wells $185.00 $0.00
480 solid wells $694.00 $0.00
480 strip wells $694.00 $0.00
Bulk Contact
Cart Total $0.00

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Please indicate any analytical testing requirements, special handling, packaging, or delivery requirements.

  • All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2015-04-01. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Estradiol EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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UltraPure Water
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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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Ann Arbor, Michigan 48108 USA

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