Estradiol EIA Kit
Item № 582251
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $185.00 $0.00
96 strip wells $185.00 $0.00
480 solid wells $694.00 $0.00
480 strip wells $694.00 $0.00

Pricing updated 2015-08-28. Prices are subject to change without notice.

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Description
Synonyms
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol
  • Estradiol ELISA Kit

Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.1 Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.1,5 Estradiol levels in most rodent samples are extremely low, rising to detectable levels only during the pro-estrus period and in pregnant females.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Download Kit Booklet Download Safety Data Sheet (SDS)

Technical Information
Synonyms
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol
  • Estradiol ELISA Kit
Limit of Detection 80% B/B0: ~19 pg/ml
Sensitivity 50% B/B0: ~129 pg/ml
Specificity
  • (100%) Estradiol
  • (14.5%) Estradiol-3-sulfate
  • (14%) Estradiol-3-glucuronide
  • (12%) Estrone
  • (10%) Estradiol-17-glucuronide
  • (0.30%) Estriol
  • (0.06%) 5α-dihydro Testosterone
  • (0.05%) Ethynyl Estradiol
  • (0.02%) 5-Androstan-17β-ol-3-one
  • (0.02%) Androstenediol
  • (<0.01%) Aldosterone
  • (<0.01%) Cortisol
  • (<0.01%) 17α-Estradiol
  • (<0.01%) Estradiol-3-benzoate
  • (<0.01%) Estradiol-17-glucoronidate
  • (<0.01%) Estradiol-17-sulfate
  • (<0.01%) Hydrocortisone
  • (<0.01%) Maturation Inducing Steroid (salmonid)
  • (<0.01%) Progesterone
  • (<0.01%) 17α-hydroxy Progesterone
  • (<0.01%) Testosterone
  • (0.008%) DHEA
  • (0.0024%) DHEA Sulfate
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WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability 1 year
Product Downloads & Resources
Product Downloads

Download Kit Booklet 684 Kb PDF

Download Abbreviated Kit Protocol 422 Kb PDF

Download Safety Data Sheet (SDS) 303 Kb PDF

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

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Inserts
QC Sheets
Certificates of Analysis
GCMS Data
References & Background Reading
Product Description References

1. Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

2. Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).

3. Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

4. Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

5. Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Background Reading

Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

Muñoz, N.M., Kim, K.P., Han, S.K., et al. Characterization of monoclonal antibodies specific for 14-kDa human group V secretory phospholipase A2 (hVPLA2). Hybridoma 19 171-176 (2000).

Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).

Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Estradiol EIA Kit

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View the pre-configured analysis tool for Estradiol EIA Kit on MyAssays.com

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