Perfecta3D® Cell Viability Kit Exclusive

Cayman Chemical Item Number 600990

Perfecta3D® Cell Viability Kit

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Description

Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. These have been routinely done in two dimensional (2D) culture plates with monolayer cells. There is a growing trend to replace 2D cell culture with 3D cell culture, because the latter better mirrors the environment experienced by normal cells in the body. One such 3D culture format is hanging drop cell culture (developed by 3D Biomatrix) in which cells form spheroids that do not come into contact with plastic. Cell spheroids grown in the hanging drops can be maintained and their growth with or without test compounds can be monitored over a period up to two weeks. Cayman’s Perfecta3D® Cell Viability Kit provides an easy to use format for studying cell proliferation in the hanging drop model using a 3D cell culture plate. The assay is based on the enzymatic cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases in viable cells. This kit will allow investigators to screen drug candidates involved in cell cycle regulation in a 3D format.
Formal Name
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Šulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).

Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

600990-1ea
WST-1 Reagent (powder) 1 ea
Electron Mediator Solution 1 ea
3D Cell Culture Plate
Round Bottom Clear Plate
600990-5ea
WST-1 Reagent (powder) 5 ea
Electron Mediator Solution 5 ea
3D Cell Culture Plate
Round Bottom Clear Plate
Size Price Quantity Subtotal
1 ea $99.00 $0.00
5 ea $249.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-24. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Perfecta3D® Cell Viability Kit under the Cayman Challenge program.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

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Manufacturer, supplier, and vendor of biochemical reagents, assay kits, forensic chemistry standards, antibodies, and proteins for scientific research · Buy now! · Analytical testing services and custom synthesis also available

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