Perfecta3D® Cell Viability Kit
Cayman Chemical Item Number 600990
Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. These have been routinely done in two dimensional (2D) culture plates with monolayer cells. There is a growing trend to replace 2D cell culture with 3D cell culture, because the latter better mirrors the environment experienced by normal cells in the body. One such 3D culture format is hanging drop cell culture (developed by 3D Biomatrix) in which cells form spheroids that do not come into contact with plastic. Cell spheroids grown in the hanging drops can be maintained and their growth with or without test compounds can be monitored over a period up to two weeks. Cayman’s Perfecta3D® Cell Viability Kit provides an easy to use format for studying cell proliferation in the hanging drop model using a 3D cell culture plate. The assay is based on the enzymatic cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases in viable cells. This kit will allow investigators to screen drug candidates involved in cell cycle regulation in a 3D format.
in continental US; may vary elsewhere
InCHI Key generation software
Šulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).
Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).
Pricing updated 2014-04-24.
Prices are subject to change without notice.
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Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample Perfecta3D® Cell Viability Kit under the Cayman Challenge program.
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