Hemoglobin Colorimetric Assay Kit

Cayman Chemical Item Number 700540

Hgb; Hb

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Limit of detection: 3 µM (0.005 g/dl) · Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Beyond binding and transporting oxygen, hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates, and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.
  • Hgb
  • Hb
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Lodemann, P., Schorer, G., and Frey, B.M. Wrong molar hemoglobin reference values-a longstanding error that should be corrected. Ann Hematol 89 209 (2010).

Allen, B.W., Stamler, J.S., and Piantadosi, C.A. Hemoglobin, nitric oxide and molecular mechanisms of hypoxic vasodilation. Trends Mol Med 15(10) 452-460 (2009).

Schechter, A.N. Hemoglobin research and the origins of molecular medicine. Blood 112(10) 3927-38 (2008).

Horton, H.R. Structure and function of biomolecules. 3 4.30-4.41 (1993 Jan 1).

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96-Well Cover Sheet
96-Well Solid Plate (Colorimetric Assay)
Hemoglobin Sample Buffer (10X) 10 mL
Hemoglobin Detector (4X) 2 × 10 mL
Hemoglobin Standard 4 × 1 mL
Size Price Quantity Subtotal
2 x 96 wells $215.00 $0.00
Bulk Contact
Cart Total $0.00

Pricing updated 2015-08-02. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Hemoglobin Colorimetric Assay Kit under the Cayman Challenge program.

Let us analyze your samples for you. See our contract research services.

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Batch-specific Information

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA


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