Hemoglobin Colorimetric Assay Kit
Item № 700540
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2 x 96 wells $215.00 $0.00

Pricing updated 2015-12-01. Prices are subject to change without notice.

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  • Assay 40 samples in duplicate
  • Measure hemoglobin from 0.016 to 0.4 g/dl
  • Measure total hemoglobin concentration in blood, tissue homogenates, and cell lysates
  • Plate-based, colorimetric measurement (560-590 nm)
  • Hgb
  • Hb

Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Beyond binding and transporting oxygen, hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates, and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS)

Technical Information
  • Hgb
  • Hb
Limit of Detection 3 µM (0.005 g/dl)

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere
Stability 1 year
Product Downloads & Resources
Product Downloads

Download Kit Booklet 397 Kb PDF

Download Safety Data Sheet (SDS) 61 Kb PDF

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

QC Sheets
Certificates of Analysis
References & Background Reading
Background Reading

Lodemann, P., Schorer, G., and Frey, B.M. Wrong molar hemoglobin reference values-a longstanding error that should be corrected. Ann Hematol 89 209 (2010).

Allen, B.W., Stamler, J.S., and Piantadosi, C.A. Hemoglobin, nitric oxide and molecular mechanisms of hypoxic vasodilation. Trends Mol Med 15(10) 452-460 (2009).

Schechter, A.N. Hemoglobin research and the origins of molecular medicine. Blood 112(10) 3927-38 (2008).

Horton, H.R. Structure and function of biomolecules. 3 4.30-4.41 (1993 Jan 1).

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Technical Support
Contact Us
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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We offer you the deep and diverse knowledge of more than 100 in-house scientists and the expertise that comes from more than three decades of designing and developing the tools to advance biomedical research.

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You may be eligible to receive a free sample of Hemoglobin Colorimetric Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA


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