Literature & Media

Characterization of Peptides Associated with Molecules of the Major Histocompatibility Complex

Application notes

Key Information

  • An optimized workflow for the sensitive and specific analysis of MHC-I peptides, using HCT116 as a case study, is presented.
  • Analysis of the enriched peptides by LC-MS/MS resulted in approximately 2,500 unique 8- to 15-mer peptides.
  • A greater than 60% enrichment of 9-mer peptides was achieved.
  • The peptide sequence data from the observed 9-mer peptides can be probed further to confirm or predict HLA allele information by motif analysis.
To cite this application note: Ford, M. Jones, R., Allen, D., et al. Characterization of peptides associated with molecules of the major histocompatibility complex. Application Note, Cayman Chemical (2017).
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Characterization of Peptides Associated with Molecules of the Major Histocompatibility Complex<br>
Cayman ChemicalCayman ChemicalCayman Chemical

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