Literature & Media

O2 Concentration of a Simulated Tumor Environment Affects Metabolic Response to Chemotherapeutics

Application notes

Key Features

  • The tumor microenvironment contains metabolically aberrant conditions that are difficult to reproduce in vitro and as a result:
    • Physiologically relevant sample types are often expensive and time consuming to obtain. 
    • Metabolic studies conducted in vitro are often carried out at ambient oxygen concentrations, which differ from that of the tumor microenvironment. 
  • HCT116 colon carcinoma cells and myeloid-derived tumor suppressor cells were cultured
    at different O2 concentrations to simulate a component of the tumor microenvironment.
  • Mitochondrial stress, fatty acid oxidation stress, and mitochondrial fuel flexibility tests reveal how metabolic phenotype can vary greatly based on culture conditions, illustrating the importance of controlling O2 concentration in metabolic studies.
To cite this application note: Hoffman, D.L. O2 Concentration of a simulated tumor environment affects metabolic response to chemotherapeutics. Application Note, Cayman Chemical (2019).
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O<sub>2</sub> Concentration of a Simulated Tumor Environment Affects Metabolic Response to Chemotherapeutics
Cayman ChemicalCayman ChemicalCayman Chemical

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