Identification and quantitation of lipids are the essential objectives of comprehensive lipidomics studies. The high number, diversity, and wide range of lipid abundances pose major challenges to the analysis of lipids by mass spectrometry and to the interpretation of complex data from biological samples. Such analyses typically require extensive and time-consuming manual curation to avoid reporting incorrect data and ensure accurate identification and quantitation.

The goal of this study was to establish a simple, robust, and versatile lipidomics LC-MS/MS workflow that increases the reliability of automated identification results and is applicable to a wide variety of biological samples. We compared lipid profiles in mouse plasma, liver tissue, and extra virgin olive oil with the workflow we developed.

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To cite this poster: Palagama, D., Gijón, M., Kennedy, P., et al. Robust and Versatile Workflow for Routine Lipidomics Analysis. Poster presented at: 70th ASMS Conference on Mass Spectrometry and Allied Topics; June 5 – 9, 2022.