This study establishes a novel LC-MS/MS method to measure tire rubber antioxidant 6-PPD, its toxic oxidized transformation product 6-PPD-Q, and several potential metabolites in fish tissue. This method can be further extended to include additional PPD compounds and metabolites and could be useful for monitoring water contamination and bioaccumulation.

To cite this technical brief: Kennedy, P.D., Goodwin, S.K., Kiewski, M.J., et al. Quantitation of 6-PPD-Q and its metabolites in fish using LC-MS/MS. Technical Brief, Cayman Chemical Company (2026).