Beyond its implications in disease, apoliporotein E (ApoE) has been implicated in the intricate process of lipid nanoparticle (LNP) uptake. Recent studies have revealed the essential role of ApoE in facilitating the cellular internalization of LNPs, providing a potential avenue for targeted drug delivery and therapeutic interventions. In this study we have expressed and purified all three ApoE isoforms and characterized their binding properties to cognate ligands by surface plasmon resonance (SPR). Furthermore, we show that Cayman-produced LNP uptake is enhanced by addition of exogenous ApoE in a cell-based assay with lung epithelial cells. 

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To cite this poster: Guerra, A.J., Muzzarelli, K.M., Gronevelt, J.P., et al. Apolipoprotein E: Purification, characterization, and lipid nanoparticle uptake enhancement. Poster presented at: PEGS Boston Summit; May 13-17, 2024. Boston, MA.