A mixture of thirteen NPS benzodiazepine standards was prepared at 200 µg/mL each and analyzed using HPLC‑PDA, with all compounds well resolved (Rs > 1.2). This streamlined method provides reliable chromatographic separation for both emerging and well established benzodiazepines and supports efficient, accurate detection in forensic and toxicological laboratories.

To cite this technical brief: Gregerson, M. Analysis of a Complex Mixture of 13 Benzodiazepines Using HPLC‑PDA Detection. Technical Brief, Cayman Chemical Company (2026).