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MACSQuant® Analyzer X Calcium flux assay in mixed populations using kinetic flow
Application notes
Key Information
Kinetic flow cytometry is an ideal approach to run a calcium flux assay on a mixed cell
Cayman’s reagents thapsigargin and fluo-3 AM can be used with Miltenyi Biotec’s recombinant antibodies and MACSQuant X Flow Cytometer to test calcium flux in multiple cell types
This simple approach can be applied to many complex cell mixes, from peripheral blood mononuclear cells (PBMCs) to transfected cells or dissociated
To cite this application note: Nyayapathy, S., Rumble, J.M. Calcium flux assay in mixed populations using kinetic flow cytometry. Application Note, Cayman Chemical (2021).
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