Literature & Media

Characterizing a Better Uncoupler

Application notes

​Key Features

  • The novel compound BAM15 uncouples oxidative phosphorylation, depleting mitochondrial membrane potential at a wider effective concentration range than the classical uncoupler FCCP.
  • BAM15 and FCCP uncoupling properties are compared in HCT116, C2C12, and Huh7 cell lines using the Seahorse XF assay to determine maximal oxygen consumption rate values in the presence of an ATP synthase inhibitor.
  • With proper uncoupler titrations, BAM15 offers more flexibility compared to FCCP when performing optimization experiments with valuable samples.
To cite this application note: Cellular Metabolism Services Group. Characterizing a better uncoupler. Application Note, Cayman Chemical (2019).
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Characterizing a Better Uncoupler<br>
Cayman ChemicalCayman ChemicalCayman Chemical

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