Literature & Media

​Chromatographic Separation of 7-Hydroxy Mitragynine from Mitragynine Pseudoindoxyl

Featured Application notes

​Key Features:

  • The co-elution of 7-hydroxy mitragynine (7-HMG; 7-OH) and mitragynine pseudoindoxyl (MP), two important mitragynine-related alkaloids, by traditional GC-MS methods makes testing a challenge for analysts.
  • If testing methods are unable to resolve these two substances in casework, identification and quantification can become compromised.
  • The rise of consumer products on the drug market containing potent semi-synthetic opioids derived from kratom, such as 7-hydroxy mitragynine and mitragynine pseudoindoxyl, is of great public health and safety concern.
  • Several chromatographic methods to achieve separation are explored in this application note. Method information presented herein may be used to assist labs in developing methods to identify and quantify 7-hydroxy mitragynine and mitragynine pseudoindoxyl in seized drug analysis and/or toxicology cases.
Developed in collaboration with Dr. Alex Krotulski, NPS Discovery, Center for Forensic Science Research and Education (CFSRE).

To cite this application note: Watson-Gooden, C., Pierzynski, H., Liu, J. et al. Chromatographic separation of 7-hydroxy mitragynine from mitragynine pseudoindoxyl. Application Note, Cayman Chemical Company (2026).
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<p>​Chromatographic Separation of 7-Hydroxy Mitragynine from Mitragynine Pseudoindoxyl<br></p>
Cayman ChemicalCayman ChemicalCayman Chemical

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