Untargeted lipidomics uses MS to analyze hundreds of molecular species in biological samples. Single-point calibration based on the known amounts of internal standards is used in many studies, especially those using shotgun (i.e., without chromatography) MS analysis, but questions remain about the accuracy of single-point calibration compared to the traditional interpolation in multipoint calibration curves, as well as its suitability in LC-MS studies, where internal standards may not coelute with most of the endogenous analytes.

The objective of this study is to assess the accuracy of quantitation of lipids in human plasma by LC-MS using either single-point or multipoint calibration curves with authentic or surrogate lipid standards, using a new preparation of human reference plasma and methods validated by comparison with published values from the NIST SRM 1950 Metabolites in Human Plasma material.

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To cite this poster: Kennedy, P.D., Palagama, D.S.W., Kwiatkowski, M.J., et al. Evaluation of the quantification capabilities of untargeted lipidomics approaches. Poster presented at: 73^rd Conference on Mass Spectrometry and Allied Topics; June 1-5, 2025; Baltimore, MD.