​Characterization of the peptides presented by MHC molecules (pMHC) on cancer cells and their immunogenic potential is key for generating anti-cancer immune responses. Experimental identification of pMHC is performed using MHC immunoprecipitation and mass spec sequencing of eluted peptides. 

In the current study, we characterize the immunopeptidome of two human glioma lines, U87 and DIPG, which were grown in nude mice. As one potential complication of cells grown in mice may be contribution of mouse pMHC to the overall peptide list, we tested specific depletion of mouse MHC prior to immunoprecipitation of human MHC complexes. We analyzed all peptide lists for the actual contribution of mouse-derived peptides to the immunopeptidome and found the true contribution of murine peptides to be minimal.

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To cite this poster: Rumble, J.M., Nyayapathy, S., Jones, R., et al. Immunopeptidome profiling of xenograft glioma samples. Poster presented at: 2023 Michigan Life Sciences Showcase; September 18, 2023; Lansing, MI.