In the present work, we demonstrate that KMN-159 stimulates osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Cells stimulated with KMN-159 in the presence of osteogenic media (OM) show increased alkaline phosphatase activity and increased mRNA levels of osteoblast phenotype markers. These changes in alkaline phosphatase activity and gene expression do not appear to be related to a simple change in cell number following KMN-159 treatment of hBM-MSCs. KMN-159 also increased the osteoclastic differentiation of RAW 264.7 cells in the presence of RANKL as shown by an increased number of TRAP positive cells.

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To cite this poster: Patel, C., Wei, S., Owen, T., et al. KMN-159, a novel EP₄ receptor agonist, stimulates osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Poster presented at: Bones and Teeth Gordon Research Conference; February 2-7, 2020; Galveston, TX.