Second messengers are small molecules that cause a wide variety of cellular changes transcriptionally, translationally, and post-translationally. 3’3’-cGAMP is a bacterial second messenger produced from ATP and GTP by specific dinucleotide cyclases. It is produced and regulated via two different pathways, which use distinct classes of synthases, effectors, and phosphodiesterases (PDEs). 3’3’-cGAMP binds to riboswitches to regulate motility, biofilm formation, virulence, and colonization through gene transcription. The study of cyclic dinucleotides (CDNs) in bacterial innate immunity is a growing area of research. Current detection methods utilize mass spectrometry, which can be both costly and timely. In addition to that, the isolation and purification of CDNs can be complicated and tedious. Rapid and accurate detection methods are critical to enable researchers to study and/or identify the relevant biological pathways.

This poster will show LC-MS/MS validation of a novel 3’3’-cGAMP monoclonal enzyme-linked immunosorbent assay (ELISA). The data provided will demonstrate the ELISA is sensitive, specific, and in good correlation with LC-MS/MS.