In this study, we formulated LNPs with SARS-CoV-2 Spike mRNA as a model antigen. The LNPs were based on established ionizable lipids, and some included putative adjuvants as additional components of the LNPs. A cohort of mice was immunized, and immune responses were assessed by ELISA for total and neutralizing antibody production, flow cytometry for T cell subsets, and ELIspot for Spike peptide-specific immune responses. We found that the different LNP formulations stimulated different aspects of the immune response, which can inform future approaches to vaccine development.

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To cite this poster: Rumble, J.M., Gronevelt, J.P., Blanks, A., et al. ​Lipid nanoparticle (LNP) components dictate the cellular immune response to vaccination. Poster presented at: The Annual Meeting of the American Association of Immunologists; May 3-7, 2025. Honolulu, HI.