In this study, LNPs encoding SARS-CoV-2 Spike protein were formulated with several different lipids proposed to stimulate immune responses. These LNPs were used to transfect human monocyte-derived dendritic cells (MDDCs), and Spike-derived MHC class I and class II peptides were identified. Evaluation of immunopeptidomic differences between these formulations as well as understanding of resulting immune responses will help the design of vaccine LNPs in the future.

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To cite this poster: Rumble, J.M., Gronevelt, J.P., Nyayapathy, S., et al. ​Lipid nanoparticle SARS-CoV-2 vaccine-mediated immunopeptidome identification. Poster presented at: The Annual Meeting of the American Association of Immunologists; May 3-7, 2025. Honolulu, HI.