In this study, we investigated the expression of three different nucleic acid cargo types (mCherry mRNA, GFP DNA and Cy5-labeled 2′3′-cGAMP) delivered to three different ovarian cancer cell lines via four loadable LNPs containing benchmark lipids SM-102, ALC-0315, (S)-C12-200, or DLin-MC3. We then compared the relative efficacy of each formulation in delivering each payload to the various ovarian cancer cells (PA-1, SK-OV-3, and Caov-3) cultured as monolayers vs spheroids to assess whether the optimal LNP formulation is consistent across 2D and 3D culture models.

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To cite this poster: Taylor, D., Forsyth, V., Riddering, C., et al. ​Lipid nanoparticle screening in 2D vs 3D cultures: Ovarian cancer cell lines exhibit different preference for optimal lipid formulation in monolayers vs spheroids. Poster presented at: American Association for Cancer Research Annual Meeting 2025; April 25-30, 2025; Chicago, IL.