Neutrophil extracellular traps (NETs) are formed from dying neutrophils expelling their contents to capture pathogens and limit the spread of infection. While they are beneficial during infection, overproduction of NETs or the inability to degrade them can be pathogenic in certain conditions, especially autoimmune disorders. Here, we describe a sensitive, versatile assay to monitor NET clearance by phagocytic cells in vitro. This assay can be performed by flow cytometry or with imaging-based platforms, which permit the kinetic monitoring of NET clearance.

These assays allow researchers to study NETosis and assess modulators of NET uptake, allowing for further studies toward treating autoimmune disorders that are driven by aberrant NETs.

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To cite this poster: Gronevelt J.P., Pisano, M., Rumble, J. Macrophage Clearance of Neutrophil Extracellular Traps (NETs). Poster presented at: IMMUNOLOGY 2022; May 6 - 10, 2022.