​Recombinant histone H3 can be citrullinated by PAD4 at 4°C with high yields at in vivo-modified sites and additional sites.

Most natively expressed proteins are modified by one or more processes that are collectively called post-translational modifications (PTMs). PTMs result in chemical and physical changes to proteins that drive essential regulatory functions. PTMs can affect thermodynamic stability, folding, activity, and interactions with other proteins and/or molecules like DNA. Citrullination is a PTM where the positively charged amino acid arginine is converted to the neutral amino acid citrulline by replacing the primary ketimine in arginine with a ketone.

Do you have a question or comment for the presenter? Let us know.

To cite this poster: Hines, J.P., Young, M.L., Assar, Z., et al. Methods in citrullination and analysis of recombinant human histones, Cayman Chemical, 2020.