Since lipids are involved in every major disease, it is impossible to overstate the importance of the use of lipid standards in their analysis as sentinels to monitor critical factors. Listen as Cayman scientist, Dr. Miguel Gijón, gives an overview of lipid biosynthetic pathways as well as a brief history of eicosanoid analysis. He also presents a typical workflow for eicosanoid analysis using LC-MS/MS, demonstrating the usefulness of lipid standards as well as recommendations for sample handling.