​Proteus mirabilis is the causative agent of up to 44% of catheter-associated urinary tract infections (UTIs). P. mirabilis has an arsenal of pathogenic strategies including swarming motility, urease production, and fimbria and biofilm formation. Cyclic dinucleotides (CDNs) play a crucial role in biofilm formation by regulating the transition between motile and stationary lifestyles via production and secretion of adhesins, polysaccharides, and DNA, all of which contribute to the stability of the extracellular matrix.

In this study, we explored the regulatory network of P. mirabilis HI4320 biofilm by utilizing BV-BRC and BioCyc databases to identify 14 genes with sequence similarity to enzymes involved in dinucleotide synthesis activity or its regulation. Investigating CDN regulation could lead to new strategies for disrupting biofilm formation and the prevention of antibiotic-resistant bacterial infections.

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To cite this poster: Forsyth, V., Owen, T., Koch, A., et al. Exploring Proteus mirabilis cyclic dinucleotide signaling network phenotypes during biofilm formation.

Poster presented at: ASM Microbe Annual Meeting 2025; June 19-23, 2025; Los Angeles, CA.