Literature & Media

Quantification of Cyclic Dinucleotides During Growth Phase in Three Bacterial Species Using ELISAs

Application notes

Key Features

  • Measurement of 3’3’-cGAMP, cyclic di-GMP, and cyclic di-AMP in bacteria samples using Cayman’s ELISAs.
  • ELISA data provides accurate and reliable results over a wide range of dilutions.
  • Bacterial cyclic dinucleotides (CDNs) can be measured in growth media and supernatants by ELISA without a cumbersome organic extraction.
  • Bacterial supernatants contain significantly higher levels of CDNs compared to corresponding lysates.
  • Different bacteria species show unique CDN profiles during growth phase in both lysates and supernatants.
  • ELISAs provide a user-friendly and cost-effective method to quantify CDN concentrations in bacteria supernatants to better understand the role these second messengers play.

To cite this application note: Metcalfe, K.C., Blaylock, J.B., Sule, G.J., et al. Quantification of Cyclic Dinucleotides During Growth Phase in Three Bacterial Species Using ELISAs. Application Note, Cayman Chemical (2023).

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Quantification of Cyclic Dinucleotides During Growth Phase in Three Bacterial Species Using ELISAs<br>
Cayman ChemicalCayman ChemicalCayman Chemical

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