​The detection and relative quantification of NET formation has relied primarily upon visualization by fluorescence microscopy, which is not accurately quantifiable, or measurement of extracellular DNA, which is quantifiable, but not exclusive to NET formation. Attempts have been made to quantify NET formation by using heterobifunctional ELISAs detecting various combinations of myeloperoxidase, neutrophil elastase, and DNA, but these suffer from a lack of reliable, reproducible standards. In addition, these components cannot serve as exclusive biomarkers for NET formation, since they are also released from neutrophil granules or during necrotic cell death. CitH3, however, can serve as a NET-specific biomarker, since it is solely produced during NET formation.

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To cite this poster: Mobley, J.L., Rumble, J.M., Domanski, P.J., et al. Quantification of neutrophil extracellular trap formation using an ELISA specific for citrullinated histone H3. Poster presented at: Neutrophil 2018 International Symposium; June 2-5, 2018; Quebec City, Quebec, Canada.