Second messengers are small molecules that cause a wide variety of cellular changes both transcriptionally, translationally, and post translationally. 3’3’-cGAMP is a bacterial second messenger produced from ATP and GTP by specific dinucleotide cyclases. It is produced and regulated via two different pathways, which use distinct classes of synthases, effectors, and phosphodiesterases (PDEs). 3’3’-cGAMP binds to riboswitches to regulate motility, biofilm formation, virulence, and colonization through gene transcription. The study of cyclic di-nucleotides (CDNs) in bacterial innate immunity is a growing area of research. Current detection methods utilize mass spectroscopy, which can be both costly and timely. In addition to that, the isolation and purification of CDNs can be complicated and tedious. Rapid and accurate detection methods are critical to enable researchers to study and/or identify the relevant biological pathways.

This poster will focus on the development and validation of a novel 3’3’-cGAMP monoclonal enzyme linked immunoassay (ELISA). The data provided will demonstrate a highly sensitive and specific ELISA validated by LC-MS/MS.

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To cite this poster: Blaylock, J., Metcalfe, K., Waters, C., and Tew, D. Robust and Development and Validation of a Novel 3’3’-cGAMP Monoclonal ELISA. Poster presented at: 28th Annual Midwest Microbial Pathogenesis Conference; September 30 – October 1, 2022.