For immunochemical detection of hepsin
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Hepsin Polyclonal Antibody (aa 241-260)

Item No. 100022

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Synthetic peptide from the internal region of human hepsin
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
IHC (formalin-fixed paraffin-embedded tissue) and WB
Species Reactivity
(+) Human
UniProt Accession №
P05981
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µl

    $347

    Add
    Cayman Chemical
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    Product Description

    Hepsin is a type II membrane-associated protein that has an extracellular proteolytic domain and exhibits low sequence homology to other known proteases.1,2 Hepsin overexpression is observed in prostate, breast, kidney, and ovarian cancers and due to low homology to other known proteases may provide a unique target for pharmacological therapy.3,4,5,6,7 Hepsin is necessary for cell growth in vitro and may play a role in metastatic expansion by factor VII activation.8,9,6 Cayman Chemical’s hepsin polyclonal antibody can be used for WB and IHC (formalin-fixed paraffin-embedded tissue) analysis for hepsin on samples of human origin. Other applications for use of this antibody have not yet been tested.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tsuji, A., Torres-Rosado, A., Arai, T., et al. Hepsin, a cell membrane-associate protease. Characterization, tissue distribution, and gene localization. The Journal of Biological Chemisty 266(25), 16948-16953 (1991).

    2. Leytus, S.P., Kurachi, K., Hagen, F.S., et al. A novel trypsin-like serine protease (hepsin) with a putative transmembrane domain expressed by human liver and hepatoma cells. Biochemistry 27(3), 1067-1074 (1988).

    3. Dhanasekaran, S.M., Barrette, T.R., Ghosh, D., et al. Delineation of prognostic biomarkers in prostate cancer. Nature 412(6849), 822-826 (2001).

    4. Magee, J.A., Araki, T., Patil, S., et al. Expression profiling reveals hepsin overexpression in prostate cancer. Cancer Res. 61(15), 5692-5696 (2001).

    5. Welsh, J.B., Sapinoso, L.M., Su, A.I., et al. Analysis of gene expression identifies candidate markers and pharmacological targets in prostate cancer. Cancer Res. 61(16), 5974-5978 (2001).

    6. Zacharski, L.R. Expression of the factor VII activating protease, hepsin, in situ in renal cell carcinoma. Thromb. Haemost. 79(4), 876-877 (1998).

    7. Tanimoto, H., Yan, Y., Clarke, J., et al. Hepsin, a cell surface serine protease identified in hepatoma cells, is overexpressed in ovarian cancer. Cancer Res. 57(14), 2884-2887 (1997).

    8. Kazama, Y., Hamamoto, T., Foster, D.C., et al. Hepsin, a putative membrane-associated serine protease, activates human factor VII and initiates a pathway of blood coagulation on the cell surface leading to thrombin formation. The Journal of Biological Chemisty 270(1), 66-72 (1995).

    9. Torres-Rosado, A., O'Shea, K.S., Tsuji, A., et al. Hepsin, a putative cell-surface protease, is required for mammalian cell growth. Proc. Natl. Acad. Sci. USA 90(15), 7181-7185 (1993).