Secondary antibody for WB or ELISA
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Product Categories

Product Type

Antibodies
Secondary Antibodies
HRP

Application

ELISA
Western Blot

Goat Anti-Rabbit IgG HRP

Item No. 10004301

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Horseradish Peroxidase-Conjugated Goat Anti-Rabbit IgG
Formulation (Request formulation change)
500 µl of immunoaffinity-purified IgG conjugated to HRP
Storage Buffer
0.01 M sodium phosphate, 0.25 M sodium chloride, pH 7.6, with 15 mg/ml BSA and 50% glycerol
Host
Goat
Origin
Animal/Goat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

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    Product Description

    This goat anti-rabbit IgG HRP is used as the 'secondary antibody' for western blotting or ELISA where the primary antibody was generated in rabbits. This peroxidase-conjugated secondary antibody is suitable for western blotting when employing detection by commercially available chemiluminescence-based substrates. Additionally this secondary antibody conjugate can be used to develop ELISAs with substrates like ABTS (2,2'-azino-bis-[3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt), OPD (O-phenylenediamine), or TMB (3,3,5,5-tetramethylbenzidine).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Buchanan, B., Meng, Q., Poulin, M.-M., et al. Comparative pharmacokinetics and safety assessment of transdermal berberine and dihydroberberine. PLos One 13(3), e0194979 (2017).

    Du, Y., Wang, L., Wu, H., et al. MicroRNA-141 inhibits migration of gastric cancer by targeting zinc finder E-box-binding homeobox 2. Mol. Med. Rep. 12(3), 3416-3422 (2015).