15-Lipoxygenase-2 Polyclonal Antibody

Two types of 15-LO have been discovered and characterized, both of which metabolize arachidonic acid (AA) to produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). 15-LO-1 oxygenates AA at both C₁₅ and C₁₂,¹ whereas 15-LO-2 exclusively oxygenates C₁₅ of AA.² Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1.^2,3 Expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues.⁴ The protein levels and enzymatic activity of 15-LO-2 are both down-regulated in prostate cancer compared with normal and benign prostate tissues, implicating a possible protective role for 15-LO-2 against tumor formation.^4,5,6 Cayman Chemical’s 15-LO-2 polyclonal antibody can be used for western blot analysis for 15-LO-2 on samples of human origin. Other applications for use of this antibody have not yet been tested.

WARNING This product is not for human or veterinary use.

1. Kuhn, H., Barnett, J., Grunberger, D., et al. Overexpression, purification and characterization of human recombinant 15-lipoxygenase. Biochim. Biophys. Acta 1169(1), 80-89 (1993).

2. Brash, A.R., Chang, M.S., and Boeglin, W.E. Discovery of a second 15S-lipoxygenase in humans. Proc. Natl. Acad. Sci. USA 94(12), 6148-6152 (1997).

3. Kilty, I., Logan, A., and Vickers, P.J. Differential characteristics of human 15-lipoxygenase isozymes and novel splice variant of 15S-lipoxygenase. Eur. J. Biochem. 266(1), 83-93 (1999).

4. Tang, S., Bhatia, B., Maldonado, C.J., et al. Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. The Journal of Biological Chemisty 277(18), 16189-16201 (2002).

5. Shappell, S.B., Boeglin, W.E., Olson, S.J., et al. 15-lipoxygenase-2 (15-LOX-2) is expressed in benign prostatic epithelium and reduced in prostate adenocarcinoma. Am. J. Pathol. 155(1), 235-245 (1999).

6. Jack, G.S., Brash, A.R., Olson, S.J., et al. Reduced 15-lipoxygenase-2 immunostaining in prostate adenocarcinoma: Correlation with grade and expression in high-grade prostatic intraepithelial neoplasia. Hum. Pathol. 31(9), 1146-1154 (2000).

Hamlett, E.D., Hjorth, E., Ledreux, A., et al. RvE1 treatment prevents memory loss and neuringlammation in the Ts65Dn mouse model of Down syndrome. Glia (2020).

Archambault, A.-S., Turcotte, C., Martin, C., et al. Comparison of eight 15-lipoxygenase (LO) inhibitors on the biosynthesis of 15-LO metabolites by human neutrophils and eosinophils. PLoS One 13(8), e0202424 (2018).

Shen, T., Shi, J., Wang, N., et al. 15-Lipoxygenase and 15-hydroxyeicosatetraenoic acid regulate intravascular thrombosis in pulmonary hypertension. Am. J. Physiol. Lung. Cell. Mol. Physiol. 309(5), L449-L462 (2015).

Yuan, D., Ran, Y., Liu, Q., et al. Enhancement of the HIF-1α/15-LO/15-HETE axis promotes hypoxia-induced endothelial proliferation in preeclamptic pregnancy. PLoS One 9(5), e96510 (2014).

Shen, T., Ma, J., Zhang, L., et al. Positive feedback-loop of telomerase reverse transcriptase and 15-lipoxygenase-2 promotes pulmonary hypertension. PLoS One 8(12), e83132 (2013).

Rhodes, L.E., Gledhill, K., Masoodi, M., et al. The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases. The FASEB Journal 23(11), 3947-3956 (2009).