CD34 Monoclonal Antibody (Clone ICO-115)

CD34 is a transmembrane phosphoglycoprotein and sialomucin protein that is commonly used as a marker for hematopoietic progenitor cells.^1,2 It is composed of an N-terminal signaling peptide, a sialylated and O-glycosylated extracellular mucin domain, a cysteine-containing globular domain, a juxtamembrane stalk region, and an intracellular C-terminal tail.³ CD34 is expressed primarily in hematopoietic progenitor cells but is also expressed in mesenchymal stromal, muscle satellite, interstitial, epithelial and vascular endothelial progenitor, and adipose mesenchymal stem/stromal cells.^1,3 It has both pro- and anti-adhesion activities, providing an anchor for L-selectin-mediated attachment of lymphocytes in vascular endothelial cells and blocking adhesion of bone marrow-derived mast cells in vitro.^1,4 CD34 has commonly been used as a marker for the selection and enrichment of hematopoietic stem cells for bone marrow transplants.¹ Cayman’s CD34 Monoclonal Antibody (Clone ICO-115) can be used for flow cytometry (FC) and immunocytochemistry (ICC) applications.

WARNING This product is not for human or veterinary use.

1. Sidney, L.E., Branch, M.J., Dunphy, S.E., et al. Concise review: Evidence for CD34 as a common marker for diverse progenitors. Stem Cells 32(6), 1380-1389 (2014).

2. Hughes, M.R., Hernaez, D.C., Cait, J., et al. A sticky wicket: Defining molecular functions for CD34 in hematopoietic cells. Exp. Hematol. 86, 1-14 (2020).

3. Scherberich, A., Di Maggio, N., and McNagny, K.M. A familiar stranger: CD34 expression and putative functions in SVF cells of adipose tissue. World J. Stem Cells 5(1), 1-8 (2013).

4. Drew, E., Merzaban, J.S., Seo, W., et al. CD34 and CD43 inhibit mast cell adhesion and are required for optimal mast cell reconstitution. Immunity 22(1), 43-57 (2005).