S1P₁ Polyclonal Antibody

Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P₁/EDG-1, S1P₂/EDG-5, S1P₃/EDG-3, S1P₄/EDG-6, and S1P₅/EDG-8.^1,2 S1P₁ primarily couples with pertussis toxin-sensitive G_i/o proteins to mediate S1P-induced cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.^1,2,3 Expression of S1P₁ is abundant in embryological vasculature and is ubiquitously expressed in adult cells suggesting diverse physiological functions of this receptor.² The human and mouse S1P₁ receptors have 382 amino acids with an estimated molecular weight of 43 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at a higher position in SDS-PAGE.⁴ Cayman’s S1P₁ Polyclonal Antibody detects the receptor at 47 kDa by WB analysis. The antibody can also be used for IF and IHC to study expression patterns of this protein.

WARNING This product is not for human or veterinary use.

1. Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J. Biochem. 131(6), 767-771 (2002).

2. Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu. Rev. Biochem. 73, 321-354 (2004).

3. Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim. Biophys. Acta 1582(1-3), 72-80 (2002).

4. Kohno, T., Wada, A., and Igarashi, Y. N-glycans of sphingosine 1-phosphate receptor Edg-1 regulate ligand-induced receptor internalization. The FASEB Journal 16, 983-992 (2002).

El-Asrar, A.M.A., Nawaz, M.I., Mohammad, G., et al. Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy. Lipids Health Dis. 13(187), (2014).